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phospho stat1 tyr701  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho stat1 tyr701
    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival <t>(STAT1,</t> STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.
    Phospho Stat1 Tyr701, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stat1+antibodies/Phospho-Stat+Antibody+Sampler+Kit/pmc12765182-83-28-33
    Average 96 stars, based on 1162 article reviews
    phospho stat1 tyr701 - by Bioz Stars, 2026-10
    96/100 stars

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    1) Product Images from "Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach"

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    Journal: Journal of Translational Autoimmunity

    doi: 10.1016/j.jtauto.2025.100341

    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.
    Figure Legend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Techniques Used: Protein-Protein interactions, Expressing, Phospho-proteomics, Control

    Related Articles

    Transfection:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Immunoprecipitation:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Chromatin Immunoprecipitation:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Incubation:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Control:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    other:

    Article Title: Role of CD44 + CCR2 + CD64 - monocyte-derived macrophage in chronic rhinosinusitis with nasal polyps.
    Article Snippet: Heterogeneity of monocyte-derived macrophages (MDMs) is gradually recognized in polyp tissue of chronic rhinosinusitis with nasal polyps (CRSwNP).. However, the contributions of MDM subsets for sustaining inflammation remain unclear.. This study therefore aimed to characterize MDM subsets in polyp tissues and estimate their functions.



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    Image Search Results


    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Journal: Journal of Translational Autoimmunity

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    doi: 10.1016/j.jtauto.2025.100341

    Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Article Snippet: Membranes were blocked with 5 % non-fat milk in TBS-T and incubated overnight at 4 °C with primary antibodies against phospho-AKT (Thr308), phospho-NFκB p65 (Ser536), phospho-p38 MAPK (Thr180/Tyr182), phospho-STAT1 (Tyr701), and phospho-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control