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stat1 d1k9y rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc stat1 d1k9y rabbit mab
    Stat1 D1k9y Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 12288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stat1+antibodies/GAPDH+Rabbit+mAb/pm42834039-574-60-64
    Average 99 stars, based on 12288 article reviews
    stat1 d1k9y rabbit mab - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Immunoprecipitation:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Chromatin Immunoprecipitation:

    Article Title: Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.
    Article Snippet: Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage.. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions.. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Incubation:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Control:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Tumor-intrinsic IFNα and CXCL10 are critical for immunotherapeutic efficacy by recruiting and activating T lymphocytes in tumor microenvironment.
    Article Snippet: .. A549 lysates were resuspended and incubated with 2 μg of control IgG, anti-IRF1, and anti-STAT1 antibodies (Cell Signaling, Danvers, Massachusetts, USA) at 4 °C overnight for immunoprecipitation. .. After incubation, the antibodies were captured by 0.25 mg of Dynabeads-Protein A (Life Technologies, Waltham, Massachusetts, USA).

    other:

    Article Title: Role of CD44 + CCR2 + CD64 - monocyte-derived macrophage in chronic rhinosinusitis with nasal polyps.
    Article Snippet: Heterogeneity of monocyte-derived macrophages (MDMs) is gradually recognized in polyp tissue of chronic rhinosinusitis with nasal polyps (CRSwNP).. However, the contributions of MDM subsets for sustaining inflammation remain unclear.. This study therefore aimed to characterize MDM subsets in polyp tissues and estimate their functions.



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    STAT3-associated changes in <t>STAT1/IRF1</t> signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of <t>p-STAT1,</t> <t>STAT1,</t> IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.
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    STAT3-associated changes in <t>STAT1/IRF1</t> signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of <t>p-STAT1,</t> <t>STAT1,</t> IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.
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    Image Search Results


    STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

    Journal: Frontiers in Physiology

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts

    doi: 10.3389/fphys.2026.1857741

    Figure Lengend Snippet: STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

    Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST).

    Techniques: Expressing, Quantitative RT-PCR, Knockdown, Western Blot, Analysis

    STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

    Journal: Frontiers in Physiology

    Article Title: Kaempferol ameliorates gestational diabetes mellitus by regulating STAT3-associated AIM2 inflammasome activation and ferroptotic injury in trophoblasts

    doi: 10.3389/fphys.2026.1857741

    Figure Lengend Snippet: STAT3-associated changes in STAT1/IRF1 signaling and AIM2 expression in HG-stimulated trophoblast cells. (A, B) RT-qPCR was performed to assess STAT3 knockdown efficiency and AIM2 mRNA expression changes in HTR-8/SVneo cells subjected to different treatments. (C–F) Western blot analysis was performed to evaluate the expression levels of p-STAT1, STAT1, IRF1, and AIM2 proteins under different experimental conditions. N = 3, not significant, ** P <0.01, *** P <0.001.

    Article Snippet: Following blocking, the membranes were incubated overnight at 4°C with primary antibodies, including AIM2 (1:1000, catalog number ab93015, Abcam), Glutathione Peroxidase 4 (GPX4, 1:1000, 52455, Cell Signaling Technology, CST), solute carrier family 7 member 11 (SLC7A11, 1:1000, 23276, CST), phospho-STAT3 (p-STAT3, 1:1000, 9145, CST), STAT3 (1:1000, 12640, CST), pro-IL-1β (1:1000, 12703, CST), IL-1β (1:1000, ab283818, Abcam), cleaved caspase-1 (1:1000, 4199, CST), pro-caspase-1 (1:1000, 24232, CST), STAT1 (1:1000, 9172T, CST), phospho-STAT1 (p-STAT1, 1:1000, 9177S, CST), IRF1 (1:1000, 9172T, CST) and β-actin (1:1000, 4967S, CST).

    Techniques: Expressing, Quantitative RT-PCR, Knockdown, Western Blot, Analysis